A number of developments spanning a multitude of techniques makes this an exciting time for research in serotonin receptors. A comprehensive review of the subject from a multidisciplinary perspective, Serotonin Receptors in Neurobiology is among the first books to include information on serotonin receptor knockout studies. With contributions from leading experts in their fields, the book explores serotonin receptors from a broad-based, multidisciplinary approach. The approaches described vary from molecular biological techniques to florescence microscopy and imaging, to genetic manipulation in animal models, providing a wide range of tools to study serotonergic phenomena.

While each of these approaches has its own advantages and limitations, the synthesis of information and knowledge achieved from studies using multiple approaches will result in a comprehensive understanding of the underlying complex phenomena involved in serotonergic signalling and its implications in health and disease. The book provides an overall understanding of these receptors based on currently used methodologies and techniques. It describes specific experimental procedures that will be of use to researchers interested in addressing similar problems involving other G-protein-coupled receptor signalling systems.

Contents

• Series Preface
• Preface
• Editor
• Contributors
• 1. Quantitative Imaging of Serotonin Autofluorescence with Multiphoton Microscopy
  S. K. Kaushalya and Sudipta Maiti.
  • INTRODUCTION
  • MULTIPHOTON MICROSCOPY
  • OPTICAL SETUP
  • IMAGING SEROTONIN AND OTHER MONOAMINES IN LIVE NEURONS
  • REFERENCES
  • ABBREVIATIONS
• 2. Monitoring Receptor-Mediated Changes of Intracellular cAMP Level by Using Ion Channels and Fluorescent Proteins as Biosensors
  Evgeni G. Ponimaskin, Martin Heine, Andre Zeug, Tatyana Voyno-Yasenetskaya, and Petrus S. Salonikidis.
  • INTRODUCTION
  • MONITORING OF RECEPTOR MEDIATED cAMP CHANGES IN LIVING CELLS BY ELECTROPHYSIOLOGICAL APPROACH
  • FÖRSTER RESONANCE ENERGY TRANSFER (FRET)-BASED cAMP SENSOR
  • REFERENCES
• 3. Membrane Organization and Dynamics of the Serotonin_1A Receptor Monitored Using Fluorescence Microscopic Approaches
  Shanti Kalipatnapu, Thomas J. Pucadyil, and Amitabha Chattopadhyay.
  • RELEVANCE OF MEMBRANE ORGANIZATION AND DYNAMICS TO PROTEIN FUNCTION
  • THE SEROTONIN_1A RECEPTOR
  • MEMBRANE ORGANIZATION OF THE SEROTONIN_1A RECEPTOR MONITORED USING DETERGENT INSOLUBILITY
  • MEMBRANE DYNAMICS OF THE SEROTONIN_1A RECEPTOR
  • CONCLUSION
  • REFERENCES
• 4. Calmodulin Is a 5-HT Receptor-Interacting and Regulatory Protein
  Justin N. Turner, Sonya D. Coaxum, Andrew K. Gelasco, Maria N. Garnovskaya, and John R. Raymond.
  • INTRODUCTION
  • 5-HT RECEPTORS AND CALMODULIN
  • CONCLUSIONS
  • REFERENCES
• 5. Identification of Novel Transcriptional Regulators in the Nervous System
  Federico Remes-Lenicov, Kirsten X. Jacobsen, Anastasia Rogaeva, Margaret Czesak, Mahmoud Hadjighasem, Mireille Daigle, and Paul R. Albert.
  • INTRODUCTION
  • IDENTIFICATION OF TRANSCRIPTION START SITE
  • PROMOTER CHARACTERIZATION
  • IDENTIFICATION OF DNA ELEMENTS
  • IDENTIFICATION OF DNA BINDING PROTEINS
  • TRANSCRIPTION FACTOR ACTIVITY
  • SUMMARY: TRANSCRIPTIONAL APPROACH TO DISCOVERY
  • REFERENCES
• 6. Serotonin 2A (5-HT_2A) Receptor Function: Ligand-Dependent Mechanisms and Pathways
  Ishier Raote, Aditi Bhattacharya, and Mitradas M. Panicker.
  • LOCALIZATION OF 5-HT_2A MRNA AND RECEPTOR PROTEIN IN THE BRAIN
  • INTRACELLULAR SIGNALING CASCADES ACTIVATED BY THE 5-HT_2A RECEPTOR
  • INTRACELLULAR LOCALIZATION OF THE 5-HT_2A RECEPTOR
  • INTRACELLULAR TRAFFICKING OF THE 5-HT_2A RECEPTOR
  • LONG-TERM REGULATION OF 5-HT_2A RECEPTOR
  • CONCLUSION
  • REFERENCES
• 7. The 5-HT_1A Receptor: A Signaling Hub Linked to Emotional Balance
  Probal Banerjee, Mukti Mehta, and Baishali Kanjilal.
  • INTRODUCTION
  • DISTRIBUTION AND ONTOGENY OF THE 5-HT_1A-R
  • PRESYNAPTIC AND POSTSYNAPTIC 5-HT_1A-R AND THEIR SIGNALING EFFECTS
  • ABERRANT 5-HT_1A-R EXPRESSION AND DISEASES
  • THERAPEUTIC AGENTS THAT FUNCTION BY REGULATING 5-HT_1A-R SIGNALING
  • RECENT ADVANCEMENT THROUGH THE USE OF THE SNRIs
  • ROLE OF 5-HT_1A-R IN THE IMMUNE SYSTEM AND CANCER
  • REQUIRED FUTURE STUDIES TO UNRAVEL MECHANISMS OF 5-HT_1A-R SIGNALING
  • EXAMPLES OF SOME STUDIES AND POSSIBLE STRATEGIES
  • CONCLUDING REMARKS
  • EXPERIMENTAL PROCEDURES
  • REFERENCES
• 8. Do Limits of Neuronal Plasticity Represent an Opportunity for Mental Diseases, Such as Addiction to Food and Illegal Drugs? Use and Utilities of Serotonin Receptor Knock-Out Mice
  Valerie Compan.
  • INTRODUCTION
  • HUMAN 5-HT RECEPTOR GENE POLYMORPHISMS AND ADDICTIONS TO FOOD AND ILLEGAL DRUGS
  • KNOCK-OUT OF 5-HT RECEPTORS CLASSICALLY REQUIRED ENVIRONMENTAL CHALLENGES TO PRODUCE MALADAPTIVE BEHAVIOR
  • 5-HT RECEPTOR KNOCK-OUT MICE AND ADAPTIVE CHANGES IN CENTRAL SEROTONINERGIC SYSTEMS
  • CONCLUSION
  • REFERENCES
• 9. Use of Mice with Targeted Genetic Inactivation in the Serotonergic System for the Study of Anxiety
  Miklos Toth.
  • ROLE OF THE 5-HT SYSTEM IN MODULATING ANXIETY STATES
  • MICE WITH TARGETED GENETIC INACTIVATION IN THE SEROTONERGIC SYSTEM FOR THE STUDY OF ANXIETY
  • BEHAVIORAL TESTS FOR ANXIETY
  • MICE WITH GENETIC INACTIVATION OF 5-HT RECEPTORS AND THE 5-HT TRANSPORTER
  • SUMMARY
  • REFERENCES

Series editors: Sidney A Simon and Miguel AL Nicolelis

This book contains information obtained from authentic and highly regarded sources. Reprinted material is quoted with permission, and sources are indicated. A wide variety of references are listed. Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materials or the consequences of their use.