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Chang SM, Matchar DB, Smetana GW, et al., editors. Methods Guide for Medical Test Reviews [Internet]. Rockville (MD): Agency for Healthcare Research and Quality (US); 2012 Jun.

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Methods Guide for Medical Test Reviews [Internet].

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This decision tree compares the treatment decisions in response to two alternative testing strategies: human epidermal growth factor receptor 2 (HER2) protein expression assay followed by HER2 gene amplification, versus HER2 protein expression assay without followup HER2 gene amplification assay. In the first instance, patients whose assay indicates that the HER2 gene is amplified have the opportunity of HER2-targeted chemotherapy. All other patients undergo surgery and adjuvant chemotherapy without the HER2-targeting agent.

Figure 2-4Add-on test example: HER2 protein expression assay followed by HER2 gene amplification assay to select patients for HER2-targeted therapy*

* Figure taken from Seidenfeld et al., 2008.15

HER2 = human epidermal growth factor receptor 2

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