we examined the link between monoallelic methylation and monoallelic expression in the
bumblebee, Bombus terrestris, by examining two whole methylome libraries and an RNA-seq library from the same bee. More...
we examined the link between monoallelic methylation and monoallelic expression in the
bumblebee, Bombus terrestris, by examining two whole methylome libraries and an RNA-seq library from the same bee. MeDIP-seq is an immunoprecipitation technique that creates libraries enriched for methylated cytosines (Harris et al., 2010). Methyl-sensitive restriction enzymes can create libraries that are enriched for non-methylated cytosines (MRE-seq) (Harris et al., 2010). Genes found in both libraries are monoallelically methylated, with the hypermethylated allele being in the MeDIP-seq data and the hypomethylated allele in the MRE-seq data (Harris et al., 2010). Monoallelic expression was identified in these loci from the RNA-seq library. If only one allele was expressed then we knew that these loci were both monoallelically methylated and monoallelically expressed in this bee. Less...