Background: Blueberry (Vaccinium spp.) is a small berry with high economic value. Although cold storage can extend the storage time of blueberry to more than 60 days, it leads to chilling injury (CI) displayed as pedicle pits; and the samples of 0°C-30 days was the critical point of chilling injury (CI). However, little is known about the mechanism and the molecular basis response to cold stress in blueberry have not been explained definitely.
Methods: To comprehensively reveal the CI mechanisms in response to cold stress, we performed high-throughput RNA Seq analysis to investigate the gene regulation network in blueberries at different storage temperatures (20°C and 0°C) in this study. At the same time, the pitting and decay rate, electrolyte leakage, malondialdehyde (MDA) and proline content were also measured.
Results: High-throughput transcriptome sequences were assembled into 35,060 unique transcripts with an N50 length of 1,348bp. A total of 1,167 up-regulated and 685 down-regulated differentially expressed genes (DEGs) were annotated and classified between the CI group and control. Forty-five cold-induced transcription factor (TF) families containing 1,023 TFs were identified. The DEGs indicated in biological processes such as stress responses; cell wall metabolism; abscisic acid, gibberellin, membrane lipid, energy metabolism, cellular components, and molecular functions were significantly responsed to low temperature storage. The transcriptional level of 40 DEGs were verified by qRT-PCR. Less...