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Conserved domains on  [gi|488904204|ref|WP_002815279|]
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thiaminase II [Leuconostoc mesenteroides]

Protein Classification

TenA family protein( domain architecture ID 14443665)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 2-215 3.39e-106

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


:

Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 304.90  E-value: 3.39e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:cd19360    1 FSEELREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWI 161
Cdd:cd19360   81 SHAHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEEGKPTPDNPFYEWI 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 488904204 162 DFYDNTDDDsevSLSDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQ 215
Cdd:cd19360  161 DFYADDEMD---GLTDQLFARLDRLAEKASEEERERAKQAFLKSCQLEWRFWEM 211
 
Name Accession Description Interval E-value
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 2-215 3.39e-106

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 304.90  E-value: 3.39e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:cd19360    1 FSEELREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWI 161
Cdd:cd19360   81 SHAHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEEGKPTPDNPFYEWI 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 488904204 162 DFYDNTDDDsevSLSDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQ 215
Cdd:cd19360  161 DFYADDEMD---GLTDQLFARLDRLAEKASEEERERAKQAFLKSCQLEWRFWEM 211
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 1-221 2.95e-66

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 203.96  E-value: 2.95e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   1 MFSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNS 80
Cdd:COG0819    2 SFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEV 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  81 EIHPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAkKQVQEKANTNDNPFKSW 160
Cdd:COG0819   82 ELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIG-KRLAERPLPPDHPYAEW 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 488904204 161 IDFYDNTDDDSEVslsDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQAYHQED 221
Cdd:COG0819  161 IETYASEEFQELV---EWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRLER 218
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 10-217 4.58e-46

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 152.35  E-value: 4.58e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   10 AAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSEIHPHHVFC 89
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   90 DVAGVEY-ETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKqVQEKANTNDNPF-KSWIDFYDNT 167
Cdd:TIGR04306  81 EVLGISDeEYFQKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATK-LKGKHTAPEHSVyHKWIETYSSS 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 488904204  168 DDDSEVSLSDKIFewiDRLAENATTQQRQQATQFFLKSCELEWQFWEQAY 217
Cdd:TIGR04306 160 WFREAVNEGENLL---NHLAETSSPEELEKLKTIFAESCEYEYNFWTMAY 206
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
2-216 5.50e-24

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 99.48  E-value: 5.50e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:PRK14713 320 FTAALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVE 399

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFcdVAGVEYETlqHATPTPKAYLyeSHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWI 161
Cdd:PRK14713 400 SELHRSW--LGDRDADT--APSPVTLAYT--DFLLARAAGGSYAVGAAAVLPCFWLYAEVGAELHARAGNPDDHPYAEWL 473

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 488904204 162 DFYdntDDDSEVSLSDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQA 216
Cdd:PRK14713 474 QTY---ADPEFAAATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQA 525
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 9-217 4.15e-22

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 90.11  E-value: 4.15e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204    9 QAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSEIHPHHVF 88
Cdd:pfam03070   3 QLRPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   89 CDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWIDFYDNTD 168
Cdd:pfam03070  83 AEALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKIRALEGPEYYEWVKTYASEY 162

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 488904204  169 DDSEVSLSDKIfewIDRLAENATTQQRQQATQFFLKSCELEWQFWEQAY 217
Cdd:pfam03070 163 FRAAVEEAKRL---LDRLLAYVSDEQLEELQEIFLKALEFEYSFWTMAL 208
 
Name Accession Description Interval E-value
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 2-215 3.39e-106

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 304.90  E-value: 3.39e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:cd19360    1 FSEELREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSDTREDMRFFLEQIGFILNGE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWI 161
Cdd:cd19360   81 SHAHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEEGKPTPDNPFYEWI 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 488904204 162 DFYDNTDDDsevSLSDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQ 215
Cdd:cd19360  161 DFYADDEMD---GLTDQLFARLDRLAEKASEEERERAKQAFLKSCQLEWRFWEM 211
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 1-221 2.95e-66

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 203.96  E-value: 2.95e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   1 MFSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNS 80
Cdd:COG0819    2 SFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEV 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  81 EIHPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAkKQVQEKANTNDNPFKSW 160
Cdd:COG0819   82 ELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIG-KRLAERPLPPDHPYAEW 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 488904204 161 IDFYDNTDDDSEVslsDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQAYHQED 221
Cdd:COG0819  161 IETYASEEFQELV---EWLIALLDRLAATASEAERERLEEAFRTASRLEYAFWDMAYRLER 218
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 2-217 3.81e-52

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 167.73  E-value: 3.81e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:cd19366    1 FSERLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNTE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWI 161
Cdd:cd19366   81 MDLHRQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAEQGGALEHNPYREWI 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 488904204 162 DFYdnTDDDSEvSLSDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQAY 217
Cdd:cd19366  161 EMY--SSDEFT-ELADWLIDLLDELAEGKSEAELERLEEIFLTSSRYEYMFWDMAY 213
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 10-217 4.58e-46

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 152.35  E-value: 4.58e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   10 AAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSEIHPHHVFC 89
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   90 DVAGVEY-ETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKqVQEKANTNDNPF-KSWIDFYDNT 167
Cdd:TIGR04306  81 EVLGISDeEYFQKIKPGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATK-LKGKHTAPEHSVyHKWIETYSSS 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 488904204  168 DDDSEVSLSDKIFewiDRLAENATTQQRQQATQFFLKSCELEWQFWEQAY 217
Cdd:TIGR04306 160 WFREAVNEGENLL---NHLAETSSPEELEKLKTIFAESCEYEYNFWTMAY 206
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 2-217 8.12e-46

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 151.57  E-value: 8.12e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:cd19364    1 FTEELREAADPLWQKSFEHPFIQGLADGTLPLETFRYYLIQDAYYLKHFAKLHALAAAKADDPAIKALLLEGAQGLAEGE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKAntnDNPF-KSW 160
Cdd:cd19364   81 LALRETFFKELGITEEEIAQTPPAPTAYHYVSHMYRQLNEGSVAEAVAALLPCYWLYQEIGERLADAGS---PVPLyQRW 157

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 488904204 161 IDFYDntDDDSEVSLSDKIfEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQAY 217
Cdd:cd19364  158 IDTYA--SDEFTESVQQQI-DLVDRLAEEASEEEREKMKQAFLISSYYELQFWEMAY 211
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 13-215 4.54e-37

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 128.62  E-value: 4.54e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  13 IMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNsEIHPHHVFCDVA 92
Cdd:cd16099    1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARALALAAAKAPDLELRTFLAELINVLDD-ELELHEKLLAEL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  93 GVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKqVQEKANtNDNPFKSWIDFYDNTDDDSE 172
Cdd:cd16099   80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPAEGLAALLPCYWSYGEIGRR-LAASLP-EHPPYRFWIDFYASDEYQEL 157

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 488904204 173 VslsDKIFEWIDRLAENATTQQRQQATQfFLKSCELEWQFWEQ 215
Cdd:cd16099  158 V---EELLQLLDQLAAAGEEEKEELKEI-FLTSLRYELMFWDA 196
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 3-216 5.45e-34

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 121.08  E-value: 5.45e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   3 SEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSEI 82
Cdd:cd19365    1 TEALWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFARFAAGAIEVER 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  83 HPHHVFCDVAGVEYETLQhaTPTPKAYLyeSHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVqeKANTNDNPFKSWID 162
Cdd:cd19365   81 ELHRSFLGEFGIDAAAEP--SPVTLAYT--SFLLATAATGPYAVAVAAVLPCFWIYAEVGKRLA--AAASPNHPYQDWID 154

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 488904204 163 FYDNTDDDSEVslsDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQA 216
Cdd:cd19365  155 TYSDEEFAEAV---RRAIAIVDRLAAEASPEERARMLEAFLRASRLEWMFWDAA 205
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 2-214 5.09e-33

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 118.45  E-value: 5.09e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:cd19361    1 LSERLYEAVEDIWDSYYEHPFVQGIADGTLDIEKFRFYMIQDYLYLLDYAKVFALGVAKAKDEEVMRFFADLINAILNEE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPF-KSW 160
Cdd:cd19361   81 MDIHRGYMKRLGITEEEIENTKPALDNLSYTSYMLSVAYEGGIAEILAAILSCSWSYEYIAKKLVERYPAALEHEFyGEW 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 488904204 161 IDFYdntDDDSEVSLSDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWE 214
Cdd:cd19361  161 VKGY---SSEEYAEANQELIDLLDRLTEDISEEQIEKLEEIFVNCSRYELKFWD 211
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 19-214 1.51e-26

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 101.54  E-value: 1.51e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  19 DHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSEIHPHHVFCDVAGVEYET 98
Cdd:cd19369    8 EHPFIKELGEGTLDKEKFKNYLIQDSLYLKEYAKVFAMGIYKSRTMKEMQFFYSSLSFVNEDETATRIKYLKEFGLTDED 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  99 LQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQE-KANTNDNPFKSWIDFYdntDDDSEVSLSD 177
Cdd:cd19369   88 IEKIEPLPENKAYTDYMLGIAKTGDVKEILMAVLPCMLSYYYIFKELVKKyKDNLESNPYKDWIEDY---ASEEYAEYCK 164

                        170       180       190
                 ....*....|....*....|....*....|....*..
gi 488904204 178 KIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWE 214
Cdd:cd19369  165 EWIDFADRLCENLSEEEKEKLKEIFRKASLYELKFWD 201
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
2-216 5.50e-24

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 99.48  E-value: 5.50e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:PRK14713 320 FTAALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVE 399

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFcdVAGVEYETlqHATPTPKAYLyeSHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWI 161
Cdd:PRK14713 400 SELHRSW--LGDRDADT--APSPVTLAYT--DFLLARAAGGSYAVGAAAVLPCFWLYAEVGAELHARAGNPDDHPYAEWL 473

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 488904204 162 DFYdntDDDSEVSLSDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQA 216
Cdd:PRK14713 474 QTY---ADPEFAAATRRAIAFVDRAFRAASPAERAAMARAFLTACRYELEFFDQA 525
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 9-217 4.15e-22

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 90.11  E-value: 4.15e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204    9 QAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSEIHPHHVF 88
Cdd:pfam03070   3 QLRPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   89 CDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWIDFYDNTD 168
Cdd:pfam03070  83 AEALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEKIRALEGPEYYEWVKTYASEY 162

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 488904204  169 DDSEVSLSDKIfewIDRLAENATTQQRQQATQFFLKSCELEWQFWEQAY 217
Cdd:pfam03070 163 FRAAVEEAKRL---LDRLLAYVSDEQLEELQEIFLKALEFEYSFWTMAL 208
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 19-217 1.35e-20

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 86.03  E-value: 1.35e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  19 DHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNsEIhPHHV-FCDVAGVEYE 97
Cdd:cd19367    8 NHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILE-EM-ELHVkYCAEWGISEE 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  98 TLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKK-QVQEKANTNDNPFKSWIDFYdntDDDSEVSLS 176
Cdd:cd19367   86 ELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRlAADPATKLEGNPYWSWIETY---ASDEYQEAV 162

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
gi 488904204 177 DKIFEWIDRLAENATTQQRQQA-TQFFLKSCELEWQFWEQAY 217
Cdd:cd19367  163 REGIELLEKLAAERPSPARLEElVKIFATATRLEIGFWDMGL 204
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 3-217 4.42e-17

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 76.59  E-value: 4.42e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   3 SEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSEI 82
Cdd:cd19363    2 TGKLRRDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLVGSTKNLSILASKAESLDLMRELLELAYGEATTEF 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  83 HPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQvQEKANTNDNP-FKSWI 161
Cdd:cd19363   82 ANYEELLDELGLSLEDAIKVEPFPTNVAYMNFLLSTSSLGSFYEGLAALLPCFWSYLEIAEYH-KDKLSENPNDiYRDWA 160

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 488904204 162 DFYDNTDddsEVSLSDKIFEWIDRLAENATTQQRQQAtqfFLKSCELEWQFWEQAY 217
Cdd:cd19363  161 SVYLSKE---YKELVERLRRIVDKYGEGEPFEKLKRI---FKTASKYEYMFWDAAY 210
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 2-217 2.92e-15

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 71.83  E-value: 2.92e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:cd19358    1 FSDRLRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFLRLLGKAVAKAPDLEAKLRLARFLGFLANDE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFCDVAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVqekANTNDNP-FKSW 160
Cdd:cd19358   81 NDYFERAFAALGVSEADREAPPLLPATRAFIDLMLEAARSGSYAEILTVLLVAEWLYLDWASRAA---AAAPLRFkHQEW 157

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 488904204 161 IDFYDNTDDDSEVslsdkifEW----IDRLAENatTQQRQQATQFFLKSCELEWQFWEQAY 217
Cdd:cd19358  158 IDLHSGPEFEAWV-------DFlrdeVDRVGPT--EEERERLEAVFARAVELEIAFFDAAY 209
PTZ00347 PTZ00347
phosphomethylpyrimidine kinase; Provisional
 13-222 1.19e-14

phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 240375 [Multi-domain]  Cd Length: 504  Bit Score: 72.30  E-value: 1.19e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  13 IMTAIHdHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSEIHPHHVFCDva 92
Cdd:PTZ00347  25 AMMSLH-LPFVQGLGDGTLDQNAFRTYIAQDTLYLNGYIRILSYCITKSDVTATGGGLLELLKGVLEELKNCHHHYID-- 101

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  93 gveyeTLQHATPTPKAYLYESHMYRA--ADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWIDFYdntdDD 170
Cdd:PTZ00347 102 -----NPDAAGPEAACRKYVDFLLASgnADTLGPSVVIAAVIPCARLYAWVGQELTNEVELTESHPFRRWLLSY----SD 172

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 488904204 171 SEVSLSDKIFE-WIDRLAENATTQQRQQAtqfFLKSCELEWQFWEQAYHQEDW 222
Cdd:PTZ00347 173 EPINTSVEQLEsLLDKYIRPGEFSEVAQA---YRRAMELEYDFFDSFGYCLGR 222
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 2-216 2.37e-14

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 71.54  E-value: 2.37e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204   2 FSEELSTQAAPIMTAIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSE 81
Cdd:PRK09517 546 FTRALWEASGDIIAEINDSDFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSSIAPDSHAQVEWAQSAAECIVVE 625

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  82 IHPHHVFCDVAGVEyetlqhATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVqeKANTNDNPFKSWI 161
Cdd:PRK09517 626 AELHRSYLSGKEAP------SAPSPVTMAYTDFLIARTYTEDYVVGVAAVLPCYWLYAEIGLMLA--EQNHDEHPYKDWL 697

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 488904204 162 DFYdntDDDSEVSLSDKIFEWIDRLAENATTQQRQQATQFFLKSCELEWQFWEQA 216
Cdd:PRK09517 698 NTY---SGEEFIAGTRAAIARVEKALENAGPEQRVDAARAFLSASVHEREFFDQA 749
TenA_C_AtTH2-like cd19368
TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine ...
 20-188 7.45e-13

TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine requiring 2; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Arabidopsis thaliana TH2 is an orphan enzyme thiamin monophosphate phosphatase which has a haloacid dehalogenase (HAD) family domain fused to its TenA_C domain, it's TenA_C domain has thiamin salvage hydrolase activity against amino-HMP. This family includes mostly uncharacterized single-domain TenA_C- like proteins; some however have additional domains such as a HAD family domain or a kinase domain It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381703 [Multi-domain]  Cd Length: 210  Bit Score: 65.34  E-value: 7.45e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  20 HAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILnSEIHPHHVFCDVAGVEYETL 99
Cdd:cd19368   19 HPFVVGLAAGNLPLDSFRHYISQDAHFLEAFARAYELAEAKADDDEDKKAIRELRKGVL-EELKLHDSYAEEWGVDLPKE 97

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204 100 QHATPTPKAY---LYESHMYRAADTGSllnILAAFQAC----PWTYNEIAKKQvqekANTNDNPFKSWIDFYdnTDDDSE 172
Cdd:cd19368   98 VTPDPATRKYtdfLLATASGKVKVAAY---TLAAMAPCmrlyAFLGQELARAL----DDTEDHPYKKWIDTY--SSQEFE 168

                        170
                 ....*....|....*.
gi 488904204 173 vSLSDKIFEWIDRLAE 188
Cdd:cd19368  169 -ALALQLEDLLDKLSA 183
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 20-216 4.85e-10

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 57.07  E-value: 4.85e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  20 HAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTdrnemkfFADSIDfILNS---------EIHpHHVFCD 90
Cdd:cd19362   18 HEFVERMRDGTLPLDNFRYYLIQDSKYVEEMLRALLRASSKAP-------LDKAIK-ILNSvfsgrdkgmEVH-KFLYSE 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  91 VaGVEYETLQHATPTPKAYLYESHMYRAADTGSLLnILAAFQACPWTYNEIAKKQVqekaNTNDNPFKSWIDFYDNTDDD 170
Cdd:cd19362   89 L-GITEDEIRRTGYNLVNYAYTRHLYYYSTLGWPQ-FLAAWAPCMWGYSEIGKYVL----NSPNELYKTWASFYASKDYK 162

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 488904204 171 SEVSLsdkIFEWIDRLAENattqqrQQATQFFLKSCELEWQFWEQA 216
Cdd:cd19362  163 KRVEA---ILEALDSIEDT------EDIKNIFRNSVNFEIMFWDAA 199
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 20-214 1.76e-07

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 50.04  E-value: 1.76e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  20 HAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSIDFILNSEIHPHHVfCDVAGVEYETL 99
Cdd:cd19359   18 NPFCQGMADGTLDLDGFGYYMVQDYYYCINYVRFKALRAAKAPDPDLLAFLAAKIKSYLDYAEDFLKT-CHIKLGIPDVV 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204 100 QHATPTPKAYLYESHMYRAADTGSLLNILAAFQACPWTYNEIAKKQVQEKANTNDNPFKSWIDfyDNTDDDSEVSLSDKI 179
Cdd:cd19359   97 DGVKPSPALKAYVDFERSVAESEDWFYLLVAMIPCIYLWYWLANQLNEDPSDKNTNFYKTWIE--PNLPDPSSAKQLEFF 174

                        170       180       190
                 ....*....|....*....|....*....|....*
gi 488904204 180 FEWidrlAENATTQQRQQATQFFLKSCELEWQFWE 214
Cdd:cd19359  175 LNA----NAAWSKIDREKANEIFRQAMQLEINFFN 205
PqqC COG5424
Pyrroloquinoline quinone (PQQ) biosynthesis protein C [Coenzyme transport and metabolism];
16-130 5.26e-04

Pyrroloquinoline quinone (PQQ) biosynthesis protein C [Coenzyme transport and metabolism];


Pssm-ID: 444176  Cd Length: 228  Bit Score: 39.88  E-value: 5.26e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 488904204  16 AIHDHAFVRGIADGYVPREALIFYVEQDFQYLSEFIKIYANAITKLTDRNEMKFFADSI---DFILNSEIHPH--HVFCD 90
Cdd:COG5424   19 YLLKHPFLQRLREGKLTREQLRAFALQRYHYVKHFPRYLAAILSRCPDEELRRALLENLyeeDGEGPEEGHIElwLRFAE 98

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 488904204  91 VAGVEYETLQHATPTPKAYLYESHMYRAADTGSLLNILAA 130
Cdd:COG5424   99 ALGLDREEVDARPPLPETRAAVDAYVNFCRRRSLLEAVAA 138
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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